NOX4 polyclonal, anti-human, mouse, rat

Background:
The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22 and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Nox4 (also known as Renox) is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. Nox4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of Nox4 produces superoxide anions. In vascular cells, the addition of Angiotensin II increases Nox4 expression, which suggests a role for Nox4 in vascular oxidative stress response. The gene encoding human Nox4 maps to chromosome 11q14.2-q21.

Alternative Name:
KOX-1, NOX4, RENOX, Kidney oxidase-1, Kidney superoxide-producing NADPH oxidase, KOX 1, KOX, Kox-1, NADPH, NADPH oxidase 4, Nox4, NOX4, Renal NAD(P)H-oxidase, RENOX,

Application Dilution:
WB:1:1,000
ICC:1:50-1:100
IHC:1:50-1:200

Specificity: NOX4 polyclonal antibody detects endogenous levels of NOX4 protein.

Immunogen:
recombinant protein

MW: 67 kDa

Swiss-Prot: Q9NPH5(Human) Q924V1(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.