NPHS2 polyclonal, anti-rat, human, mouse

Quantity: 100 µg

Background:
Podocin(PDCN) is a protein which lines the podocytes and assists in maintaining the barrier at the glomerular basement membrane. NPHS2 is a causative gene for Familial idiopathic nephrotic syndromes, which represents a heterogeneous group of kidney disorders, and include autosomal recessive steroid-resistant nephrotic syndrome, which is characterized by early childhood onset of proteinuria, rapid progression to end-stage renal disease and focal segmental glomerulosclerosis. By positional cloning, NPHS2 was mapped to 1q25-31. It is almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli, and encodes a new integral membrane protein, podocin, belonging to the stomatin protein family. Boute et al.(2000) found ten different NPHS2 mutations, comprising nonsense, frameshift and missense mutations, to segregate with the disease, demonstrating a crucial role for podocin in the function of the glomerular filtration barrier.

Description:
Rabbit IgG polyclonal antibody for Podocin (NPHS2) detection. Tested with WB in Human, Mouse, Rat (Kidney).

Synonyms:
Nephrosis 2, idiopathic, steroid resistant(podocin) antibody, nephrosis 2, idiopathic, steroid resistant antibody, NPHS2 antibody, NPHS2 gene antibody, PDCN antibody, PODO_HUMAN antibody, Podocin antibody, SRN1 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence in the middle region of human NPHS2(126-143aa CVKVVQEYERVIIFRLGH), different from the mouse sequence by one amino acid.A synthetic peptide corresponding to a sequence in the middle region of human NPHS2(126-143aa CVKVVQEYERVIIFRLGH), different from the mouse sequence by one amino acid.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: rat.
Predicted to work with: human, mouse.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.