NPM (Phospho-Thr234) polyclonal, anti-human, mouse, rat

Specificity:
NPM (Phospho-Thr234) Antibody detects endogenous levels of NPM1 only when phosphorylated at Thr234.

Synonyms: B23.1, NPM isoform 1, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, nucleophosmin, nucleophosmin (Phospho-nucleolar phosphoprotein B23, numatrin), Numatrin

Immunogen:
The antiserum was produced against synthesized phospho-peptide derived from human NPM around the phosphorylation site of Thr234.

Immunogen Sequence:
Phospho

Modification Sites: H:T234, M:T232, R:T232

MW (kDa): 32 kDa

NCBI Gene Symbol: NPM1

Database Links:
Gene ID: 4869
Swiss Prot No.: P06748
OMIM No.: 164040
Unigene No.: Hs.557550/Hs.654469/Hs.654583

Application Dilutions: WB: 1:500~1:1000, ELISA: 1:10000

Purification:
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Physical Format:
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Concentration: 1 mg/ml

Storage: Stable at -20°C for at least 1 year.