NR3C1 Polyclonal Antibody

Background:
This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175).

Research Area: Cancer, Epigenetics and Nuclear Signaling, Signal Transduction

Synonyms:
GCCR, GCR, GCRST, GR, GRL, NR3C1

Immunogen: Recombinant fusion protein of human NR3C1 (NP_001191194.1).

Swissprot: P04150
Gene ID: 2908

Calculated MW: 49-85 kDa
Observed MW: 86 kDa/96 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Concentration: 1 mg/mL
Dilution: "WB 1:500-1:2000 IHC 1:50-1:200"

Storage:
Store at -20°C. Avoid freeze / thaw cycles.