NRF1 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
NRF1(Nuclear Respiratory Factor 1), also known as Alpha-Pal. Gopalakrishnan and Scarpulla(1995) analyzed DNA from a panel of human/hamster cell hybrids using human-specific NRF1 PCR primers and localized the NRF1 gene to human chromosome 7. The assignment was further refined to 7q31 by cohybridization of NRF1- and chromosome 7-specific probes to human metaphase chromosomes. Efiok et al.(1994) identified genes containing alpha-Pal-binding sequences and found that these could be classified either as cellular proliferation genes, or as genes regulating the growth-responsive metabolic pathways of energy transduction, translation, and replication. Virbasius and Scarpulla(1994) noted that the nuclear-encoded mitochondrial transcription factor TFAM contains potential binding sites for NRF1, NRF2(GABPA) and SP1 within the promoter region. With use of binding and electrophoretic mobility shift assays, DNase footprinting, and mutation analysis of recombinant proteins, they demonstrated specific and functional binding of NRF1 and NRF2 to the TFAM promoter region.

Description:
Rabbit IgG polyclonal antibody for Nuclear respiratory factor 1 (NRF1) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.

Synonyms:
alpha pal antibody, alpha palindromic binding protein antibody, Alpha palindromic-binding protein antibody, Alpha-pal antibody, locus control region factor 1 antibody, NFE2 related factor 1 antibody, NRF-1 antibody, Nrf1 antibody, NRF1_HUMAN antibody, nuclear factor(erythroid derived 2)-like 1 antibody, nuclear respiratory factor 1 antibody, transcription factor 11 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence in the middle region of human NRF1(272-288aa QHGREDLLYAFEDQQTQ), identical to the related rat and mouse sequences.A synthetic peptide corresponding to a sequence in the middle region of human NRF1(272-288aa QHGREDLLYAFEDQQTQ), identical to the related rat and mouse sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB, IHC-P, ICC

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.