nSMase3 (C-terminal region) Blocking Peptide
€155.00
In stock
SKU
ECM-SX0285
Background:
Cellular stress leads to hydrolysis of sphingomyelin to generate lipid second messenger molecules including ceramide, sphingosine, and sphingosine-1-phosphate. A variety of sphingomyelinase activities have been described that differ in tissue and subcellular distribution, as well as pH and cation dependence. These enzymes generate ceramide for specific signaling pathways that lead to a wide range of cellular responses, such as apoptosis, cell cycle arrest, cell survival, and cell proliferation. Neutral sphingomyelinases (nSMases) are Mg2+-dependent neutral pH SMases, and the family includes nSMase1, nSMase2, and nSMase3. These nSMases differ in their tissue distribution and subcellular localization. nSMase3 (also known as SMPD4) is highly expressed in skeletal muscle and heart tissues, and is expressed in many cancer cell lines. The nSMase3 protein is localized to endoplasmic reticulum and Golgi, and may be upregulated in response to DNA damage and cell stress.
Background References
Wiegmann, K.O. et al. (2006) J Biol Chem. 281(19):13784.
Corcoran, C.A. et al. (2008) Mol Can Res. 6(5):795.
Sequence: nSMase3 synthetic peptide corresponds to amino acids in the C-terminal region of mouse nSMase3. This sequence is highly conserved in rat and human nSMase3, and has low homology to other nSMase family members.
Specificity: The peptide is specifically recognized by nSMase3 (C-terminal region) antibody (SP0281) in ELISA, and has been shown to block the reactivity of SP0281 in Western blot. In addition, the peptide is recommended for use in blocking SP0281 reactivity in immunocytochemistry.
Buffer/Storage:
Blocking Peptide is supplied in 50 µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Cellular stress leads to hydrolysis of sphingomyelin to generate lipid second messenger molecules including ceramide, sphingosine, and sphingosine-1-phosphate. A variety of sphingomyelinase activities have been described that differ in tissue and subcellular distribution, as well as pH and cation dependence. These enzymes generate ceramide for specific signaling pathways that lead to a wide range of cellular responses, such as apoptosis, cell cycle arrest, cell survival, and cell proliferation. Neutral sphingomyelinases (nSMases) are Mg2+-dependent neutral pH SMases, and the family includes nSMase1, nSMase2, and nSMase3. These nSMases differ in their tissue distribution and subcellular localization. nSMase3 (also known as SMPD4) is highly expressed in skeletal muscle and heart tissues, and is expressed in many cancer cell lines. The nSMase3 protein is localized to endoplasmic reticulum and Golgi, and may be upregulated in response to DNA damage and cell stress.
Background References
Wiegmann, K.O. et al. (2006) J Biol Chem. 281(19):13784.
Corcoran, C.A. et al. (2008) Mol Can Res. 6(5):795.
Sequence: nSMase3 synthetic peptide corresponds to amino acids in the C-terminal region of mouse nSMase3. This sequence is highly conserved in rat and human nSMase3, and has low homology to other nSMase family members.
Specificity: The peptide is specifically recognized by nSMase3 (C-terminal region) antibody (SP0281) in ELISA, and has been shown to block the reactivity of SP0281 in Western blot. In addition, the peptide is recommended for use in blocking SP0281 reactivity in immunocytochemistry.
Buffer/Storage:
Blocking Peptide is supplied in 50 µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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