Nuclei (clone 235-1), anti-human
€442.00
In stock
SKU
253197
Catalog Nr.: 253197
Size: 0.1 ml
Isotype: Mouse IgG1
Applications: IF, IHC, E
Datasheet
Request Information
Size: 0.1 ml
Isotype: Mouse IgG1
Applications: IF, IHC, E
Datasheet
Request Information
Protein Family: Transcription Factors
Pathway and Disease: Transcription
Description:
The nuclei antibody is an excellent marker for human cells in xenographic model research. The nuclei antibody reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells. This antibody stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. The antibody works on acetone or paraformaldehyde-fixed cells. Cell permeabilization with 0.1-0.4% Triton X100 is recommended. The antibody was positively tested on paraformaldehyde-fixed cryostat sections for immunohistochemistry.
Alternate Names: Human cell nucleus, nuclei
Application Notes: E: 1:500-1:1,000; IF: 1:100; IHC: 1:100
Antigen: Human cell homogenate.
Format: Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of HEPES (pH7.5), 0.01% BSA, with 50% glycerol. Antibody was purified by Protein-G affinity chromatography.
Storage:
Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment.
Pathway and Disease: Transcription
Description:
The nuclei antibody is an excellent marker for human cells in xenographic model research. The nuclei antibody reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells. This antibody stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. The antibody works on acetone or paraformaldehyde-fixed cells. Cell permeabilization with 0.1-0.4% Triton X100 is recommended. The antibody was positively tested on paraformaldehyde-fixed cryostat sections for immunohistochemistry.
Alternate Names: Human cell nucleus, nuclei
Application Notes: E: 1:500-1:1,000; IF: 1:100; IHC: 1:100
Antigen: Human cell homogenate.
Format: Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of HEPES (pH7.5), 0.01% BSA, with 50% glycerol. Antibody was purified by Protein-G affinity chromatography.
Storage:
Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment.
| Is Featured? | No |
|---|
Write Your Own Review