Nuf2 polyclonal, anti-human

Background:
This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.

Alternative Name:
hNuf2, hNuf2R, hNuf2R, Cancer/testis antigen 106, CDCA 1, CDCA1, Cell division cycle associated 1, Cell division cycle associated protein 1, Cell division cycle-associated protein 1, CT106, hNuf 2, hNuf2, hNuf2R, hsNuf2, Kinetochore protein Nuf2, Nuf 2, NUF2, NUF2 NDC80 kinetochore complex component, NUF2 NDC80 kinetochore complex component homolog, NUF2, S. cerevisiae, homolog of, NUF2, NUF2R,

Application Dilution:
WB:1:500-1:2,000
FC:1:50-1:100

Specificity: Nuf2 polyclonal antibody detects endogenous levels of Nuf2 protein.

Immunogen:
Recombinant protein within human Nuf2 aa 1-200.

MW: 54 kDa

Swiss-Prot: Q9BZD4(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.