NuMA (clone EBS-C-002), anti-human

Source:
An NZB mouse was immunized with live Ls 174T cells (colon carcinoma).
Fusion partner: X63-Ag8.653.

Specifications:
EBS-C-002 reacts with NuMA or Nuclear Mitotic Apparatus protein, which at the onset of mitosis redistributes from the nucleus to two centrosomal structures at the poles of the mitotic spindle, where it plays a vital role in establishing and maintaining its bipolar structure. After anaphase the protein redistributes from the spindle polar region into the reforming nucleus and concentrates initially at the site where nuclear lamins and perichomatin have been reported to assemble. In contrast to mitotic cells, post-mitotic neurons display NuMA both in the nucleus and in the cytoplasm. Due to release from dead cells, NuMA is also used as oncological marker in serum and urine. In addition, chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.

Species reactivity:
Positive: human.

Applications:
EBS-C-002 can be applied to investigate mitosis in tissue sections. With mitotic cells on Western blot a main band at 210 kDa is shown and additional weaker bands at 240 and 180 kDa. In ELISA EBS-C-002 can be used for oncological serum (a.o. colonic cancer) and urine tests (bladder cancer).
ELISA: +
Frozen sections: +
Paraffin sectionsTris/EDTA
Western blot: +

Positive control: Human toncil.

References:
- Butschak, G. et al., Acta Histochem.97: 19-31 (1995).
- Compton, D.A. et al., J.Cell Biol.116: 1395-1408 (1992)