Occludin polyclonal, anti-human, mouse, rat

Background:
Occludin is an integral membrane protein closely associated with the tight junctions of epithelial and endothelial cells. Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of occludin. Expression of occludin is up-regulated by poly-unsaturated fatty acids, increasing transendothelial cell resistance and reducing cellular permeability to large molecules. The level of occludin varies greatly depending on tissue, in brain tissue, occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Up-regulation of epithelial occludin may play a role in enhancing paracellular permeability and be related to the damage to the tight junction.

Alternative Name:
OCLN, BLCPMG, FLJ08163, FLJ18079, FLJ77961, FLJ94056, MGC34277, Occludin, Ocln, OCLN, Tight junction protein occludin,

Application Dilution:
WB:1:1,000
FC:1:50-1:100

Specificity: Occludin polyclonal antibody detects endogenous levels of Occludin protein.

Immunogen:
recombinant protein

MW: 59 kDa

Swiss-Prot: Q16625(Human) Q61146(Mouse) Q6P6T5(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.