OPG/TNFRSF11B mouse recombinant protein

Background:
Tnfrsf11b, also known as tumor necrosis factor receptor superfamily member 11B, is a member of the tumor necrosis factor receptor TNFR superfamily. It inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Also It plays a role in preventing arterial calcification. Recombinant mouse Tnfrsf11B, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names:
Tumor necrosis factor receptor superfamily member 11B isoform 1, Osteoclastogenesis inhibitory factor, Osteoprotegerin, OCIF, OPG, TR1

Domain: 22-401aa
Sequence:
ETLPPKYLHY DPETGHQLLC DKCAPGTYLK QHCTVRRKTL CVPCPDHSYT DSWHTSDECV YCSPVCKELQ SVKQECNRTH NRVCECEEGR YLEIEFCLKH RSCPPGSGVV QAGTPERNTV CKKCPDGFFS GETSSKAPCI KHTNCSTFGL LLIQKGNATH DNVCSGNREA TQKCGIDVTL CEEAFFRFAV PTKIIPNWLS VLVDSLPGTK VNAESVERIK RRHSSQEQTF QLLKLWKHQN RDQEMVKKII QDIDLCESSV QRHLGHSNLT TEQLLALMES LPGKKISPEE IERTRKTCKS SEQLLKLLSL WRIKNGDQDT LKGLMYALKH LKTSHFPKTV THSLRKTMRF LHSFTMYRLY QKLFLEMIGN QVQSVKISCL

UniProt No.: O08712
NCBI Accession No.: NP_032790

Molecular Weight: 44.4 kDa (388aa)
Concentration: 0.25mg/ml (determined by absorbance at 280nm)
Formulation: Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Purity: > 90% by SDS-PAGE
Bioactivity: Measured by its ability to inhibit cytotoxicity using Jurkat human acute T cell leukemia cells in the presence of 2 ng/ml of human TRAIL (CAT# ATGP3588). The ED50 range ≤ 50 ng/ml.
Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
Endotoxin: His-Tag
Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.