Orai1 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
ORAI1(ORAI calcium release-activated calcium modulator 1), also known as CRACM1, TMEM142A, Calcium release-activated calcium channel protein 1, Protein orai-1, Transmembrane protein 142A, FLJ14466, is a calcium selective ion channel that in humans is encoded by the ORAI1 gene. Orai1 channels play an important role in the activation of T-lymphocytes. The loss of function mutation of Orai1 causes severe combined immunodeficiency(SCID) in humans. The mammalian orai family has two additional homologs, orai2 and orai3. Orai proteins share no homology with any other ion channel family of any other known proteins. They have 4 transmembrane domains and form tetramers. Prakriya et al.(2006) showed that ORAI1 is a PM protein, and that CRAC channel function is sensitive to mutation of 2 conserved acidic residues in the transmembrane segments. Glu106-to-asp(E106D) and glu190-to-gln(E190Q) substitutions in transmembrane helices 1 and 3, respectively, diminished calcium ion influx, increased current carried by monovalent cations, and rendered the channel permeable to cesium ion. Prakriya et al.(2006) showed that ORAI1 is a PM protein, and that CRAC channel function is sensitive to mutation of 2 conserved acidic residues in the transmembrane segments.

Description:
Rabbit IgG polyclonal antibody for Calcium release-activated calcium channel protein 1 (ORAI1) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
Calcium release activated calcium channel protein 1 antibody, Calcium release activated calcium modulator 1 antibody, Calcium release-activated calcium channel protein 1 antibody, CRACM 1 antibody, CRACM1 antibody, CRCM1 antibody, CRCM1_HUMAN antibody, FLJ14466 antibody, Orai 1 antibody, ORAI calcium release activated calcium modulator 1 antibody, orai1 antibody, ORAT1 antibody, Protein orai 1 antibody, Protein orai-1 antibody, TMEM 142A antibody, TMEM142A antibody, Transmembrane protein 142A antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human Orai1(278-301aa EFARLQDQLDHRGDHPLTPGSHYA), different from the related rat and mouse sequences by two amino acids.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human.
Predicted to work with: mouse, rat.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.