ORAI3 (clone 2H2G9), anti-human, rat

Protein Family: Receptors and Channels

Pathway and Disease: Immune System, Signal Transduction

Description:
Antigen stimulation of immune cells triggers Ca++ entry t hrough Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2. Larger molecular weight bands are sometimes seen in SDS-PAGE; these may represent post-translationally modified ORAI 3.

Alternate Names: ORAI3, Transmembrane protein 142C, TMEM142C, Calcium release-activated calcium channel protein 3

Application Notes: E: 1:500-1:1,000; WB: 1:100-1:500; IHC: 1:100-1:500

Accession No.: Q9BRQ5

Antigen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ORAI3.

Format: Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.02% sodium azide. Antibody was purified by Protein-A affinity chromatography.

Storage:
Store at 4°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment.