Osteocrin human recombinant protein

Background:
Osteocrin, also known as Musclin, shows a well conserved homology with members of the natriuretic peptide (NP) family. It is a secreted protein that is expressed in muscle and bone. Based on similarities with NPs, Osteocrin could interact with the NP clearance receptors, importantly increasing CNP which has been shown to stimulate endochondral ossification and elongate bones. It is represents a novel, unique vitamin D-regulated bone-specific. Recombinant human Osteocrin, fused to His-tag at C-terminus, was expressed in HEK 293 cell and purified by using conventional chromatography techniques.

Alternative Names:
Osteocrin, MUSCLIN, OSTN

Domain: 28-133aa
Sequence:
VDVTTTEAFD SGVIDVQSTP TVREEKSATD LTAKLLLLDE LVSLENDVIE TKKKRSFSGF GSPLDRLSAG SVDHKGKQRK VVDHPKRRFG IPMDRIGRNR LSNSRG

UniProt No.: P61366
NCBI Accession No.: NP_937827.1

Molecular Weight: 12.5kDa (112aa)
Concentration: 0.5mg/ml (determined by Bradford assay)
Formulation: Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Purity: > 95% by SDS-PAGE
Bioactivity: Measured by its binding ability in a functional ELISA with Human NPRC. The ED50 range ≤ 20 ng/ml.
Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
Endotoxin: His-Tag