Osteopontin polyclonal, anti-human

Quantity: 100 µg

Background:
Osteopontin(OPN) also called urinary stone protein, secreted phosphoprotein 1(SPP1), bone sialoprotein, and early T lymphocyte activation 1(ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein(34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4. The standard product used in this kit is recombinant human OPN, consisting of 17-314 amino acid sequence with the molecular mass of 32.9KDa. As a result of glycosylation, the molecular mass is 60-65KDa.

Description:
Rabbit IgG polyclonal antibody for Osteopontin (SPP1) detection. Tested with WB, IHC-P in Human.

Synonyms:
BNSP antibody, Bone sialoprotein 1 antibody, BSP I antibody, BSPI antibody, Early T lymphocyte activation 1 antibody, ETA 1 antibody, ETA1 antibody, MGC110940 antibody, Nephropontin antibody, OPN antibody, Osteopontin antibody, osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody, OSTP_HUMAN antibody, PSEC0156 antibody, secreted phosphoprotein 1(osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) antibody, Secreted phosphoprotein 1 antibody, SPP 1 antibody, SPP-1 antibody, SPP1 antibody, SPP1/CALPHA1 fusion antibody, Urinary stone protein antibody, Uropontin antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of human Osteopontin(28-47aa EEKQLYNKYPDAVATWLNPD), different from the related mouse sequence by six amino acids.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.