P14ARF polyclonal, anti-mouse, rat

Protein Family: Chaperones and Folding Catalysts

Pathway and Disease: Cell Growth and Death, Cancers

Description:
Cyclin-dependent kinase inhibitor 2A (P14ARF, or p19ARF in mouse) is capable of inducing cell cycle arrest in G1 and G2 phases. P14ARF acts as a tumor suppressor. P14ARF binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. P14ARF also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes.

Alternate Names: Cyclin-dependent kinase inhibitor 2A, isoform 4, p14ARF, p19ARF, CDKN2A, CDKN2, MLM

Application Notes: E: 1:500-1:1,000; WB: 1:100-1:500; IHC: 1:100-1:500

Accession No.: Q8N726

Antigen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of mouse p14/19ARF.

Format: Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4, 25% glycerol, 1 mg/ml BSA with 0.09% sodium azide. Antibody was purified by Protein-A affinity chromatography.

Storage:
Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment.

Product Citations:
Yang Y-L et al. 2014. J Pathol. 233(2): 113-23. PMID 24648314.