p16 INK4a/CDKN2A polyclonal, anti-human

Background:
The progression of cells through the cell cycle is regulated by a family of proteins designated cyclin-dependent kinases (Cdks). Sequential activation of individual members of this family and their consequent phosphorylation of critical substrates, promote orderly progression through the cell cycle. The protein p16INK4A, identified as a negative regulator of the cell cycle, has been shown to bind to and inhibit the activity of the Cdk4/cyclin D complex. p19 ARF, which is unrelated to p16, arises from transcription of an alternative reading frame of the p16 gene. Like p16, p19 ARF has been shown to induce cell cycle arrest. Mice lacking p19 ARF but expressing functional p16 have been shown to develop tumors early in life. Further studies have indicated that p19 ARF may be disrupted in a large percentage of human T cell acute lymphoblastic leukemias.

Alternative Name:
CDK4I, MTS-1, p16-INK4, p16INK4A, CCM2, CDK4 inhibitor p16 INK4, CDK4I, CDKN2, CDKN2A, Cell cycle negative regulator beta, CMM2, Cyclin dependent kinase 4 inhibitor A, Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4), Cyclin Dependent Kinase Inhibitor 2A, Cyclin dependent kinase inhibitor 2A isoform 4, Cyclin dependent kinase inhibitor 2A isoforms 1/2/3, Cyclin dependent kinase inhibitor p16, INK4, INK4A, MLM, MTS1, Multiple tumor suppressor 1, p14, p16, P16INK4, p16INK4a, p19, p19Arf, TP16,

Application Dilution:
WB:1:500-1:1,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Specificity: p16 INK4a/CDKN2A polyclonal antibody detects endogenous levels of p16 INK4a/CDKN2A protein.

Immunogen:
recombinant protein

MW: 17 kDa

Swiss-Prot: P42771(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.