P21 Polyclonal Antibody

Background:
May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739).

Research Area: Cell Biology, Epigenetics and Nuclear Signaling, Signal Transduction

Synonyms:
CDKI, CDKN1A-interacting zinc finger protein 1, Cip1-interacting zinc finger protein, CIZ1, CIZ1, cyclin dependent inhibitor 1A, LSFR1, Melanoma differentiation-associated protein, NP94, Nuclear protein NP94, OPHN3, P21, Zinc finger protein 356, ZNF356

Immunogen: Recombinant Human P21 protein

Swissprot: P38936
Gene ID: 1026

Calculated MW: 18 kDa
Observed MW: 18 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.05% Proclin300 and 50% glycerol, pH7.4.

Concentration: 2 mg/mL
Dilution: WB 1:500-1:1000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Alternative for product with catalog number:
sc-471,sc-469,sc-397,sc-756