p27 Kip1 polyclonal, anti-human, mouse, rat
€388.00
In stock
SKU
BS6465
Background:
Cell cycle progression is regulated by a series of cyclin-dependent kinases that consist of catalytic subunits, designated Cdks, and activating subunits, designated cyclins. Orderly progression through the cell cycle requires the activation and inactivation of different cyclin-Cdks at appropriate times. A series of proteins has been recently described that function as “mitotic inhibitors.” These include p21, the levels of which are elevated upon DNA damage in G1 in a p53-dependent manner, p16 and a more recently described p16 related inhibitor designated p15. A p21 related protein, p27, has been described as a negative regulator of G1 progression and has been speculated to function as a possible mediator of TGFβ-induced G1 arrest. p27 interacts strongly with D-type cyclins and Cdk4 in vitro and to a lesser extent with cyclin E and Cdk2.
Alternative Name:
Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor, p27, p27Kip1, CDKN1B, KIP1
Application Dilution: IHC: 1:50 - 1:200, IF: 1:20 - 1:50
Specificity: p27 Kip1 polyclonal antibody detects endogenous levels of p27 Kip1 protein.
Immunogen:
Recombinant full length Human p27 Kip1.
MW: ~ 22 kDa
Swis Prot.: P46527
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
Cell cycle progression is regulated by a series of cyclin-dependent kinases that consist of catalytic subunits, designated Cdks, and activating subunits, designated cyclins. Orderly progression through the cell cycle requires the activation and inactivation of different cyclin-Cdks at appropriate times. A series of proteins has been recently described that function as “mitotic inhibitors.” These include p21, the levels of which are elevated upon DNA damage in G1 in a p53-dependent manner, p16 and a more recently described p16 related inhibitor designated p15. A p21 related protein, p27, has been described as a negative regulator of G1 progression and has been speculated to function as a possible mediator of TGFβ-induced G1 arrest. p27 interacts strongly with D-type cyclins and Cdk4 in vitro and to a lesser extent with cyclin E and Cdk2.
Alternative Name:
Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor, p27, p27Kip1, CDKN1B, KIP1
Application Dilution: IHC: 1:50 - 1:200, IF: 1:20 - 1:50
Specificity: p27 Kip1 polyclonal antibody detects endogenous levels of p27 Kip1 protein.
Immunogen:
Recombinant full length Human p27 Kip1.
MW: ~ 22 kDa
Swis Prot.: P46527
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
| Is Featured? | No |
|---|
Write Your Own Review