p34 (clone POH-1), anti-human, bovine, mink, monkey

Source:
A BALB/c mouse was immunized with recombinant human p34cdc2 fusion protein.
Fusion partner: NS-2.

Specifications:
POH-1 detects the three bands within the 34kDa region corresponding to the p34 protein cyclin dependent kinase 1 (cdk1) and its cleavage products. It is positive in immunoblotting on HeLa cell lysate and negative on fibroblast (LEP strain) cell lysate. A cdc2 homolog, the cdk2 protein kinase, does not react with POH-1. Activated cdk1 / p34cdc2 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.

Species reactivity:
Positive: cow, human, mink, monkey. Negative: drosophila, mouse, rat, Saccharomyces cerevisiae, Schizosaccharomyces pombe.

Applications:
POH-1 can be used in immunohistochemistry, fluorescence tests and Western blotting.
ELISA: +
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate
sWestern blot: +

Positive control: HeLa, MCF7.

References:
- Lukáš, J, et al. Eur. J. Biochem., 207: 169-176 (1992)