p38 MAP Kinase (Thr-180/Tyr-182), phospho-specific (clone M139), anti-human, mouse, rat

Background:
p38 MAP kinase (MAPK), also called RK, CSBP, and SAPK2a, is the mammalian orthologue of the yeast HOG kinase. This family of kinases participates in signaling cascades that control cellular responses to cytokines and stress. Four isoforms of p38 MAPK (α,β,γ,δ) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides, UV light, and growth factors. MKK3 and SEK activate p38 MAPK by dual phosphorylation at threonine 180 and tyrosine 182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 and to phosphorylate the transcription factors ATF-2, Max and MEF2.

Immunogen: Clone M139 was generated from a phospho-p38α (Thr-180/Tyr-182) synthetic peptide (coupled to KLH) corresponding to amino acid residues around threonine 180 and tyrosine 182 of rat p38α. This peptide sequence is highly conserved in the p38β,g, and d MAPKs, and is identical in human and mouse p38α.

Specificity: This antibody detects a 38 kDa* protein corresponding to the apparent molecular mass of p38α on SDS-PAGE immunoblots of pervanadate treated human Jurkat and A431 cells, as well as anisomycin treated human HeLa cells.

Buffer/Storage:
Mouse monoclonal purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.