p53 (clone Bp53-12), anti-human, chicken, dog, hamster, monkey

Source:
A BALB/c mouse was immunized with recombinant human p53 protein.
Fusion partner: P3-X63-Ag8.653.

Specifications:
Bp53-12 reacts with an N-terminal epitope (aa 16-25) of both wild-type and mutated p53. This epitope is revealed in tissue sections only after formalin fixation. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution.

Species reactivity:
Positive: chicken, dog, hamster, human, monkey. Negative: mouse, rat.

Applications:
Bp53-12 is not suitable for frozen sections. Only formalin fixed paraffin embedded tissue section should be used. Bp53-12 also works well in fluorescence tests and Western blots.
Flow cytometry: +
Frozen sections: -
Immunofluorescence: +
Paraffin section: Citrate
Western blot: +

Positive control: Paraffin sections of normal human breast. MCF7 or A431 cell lysates.

References:
- Bártek J. et al,J Pathol.169(1):27-34 (1993).
- Vogelstein and Kinzler, Cell70: 523-526, (1992).
- Hollstein et al, Science 253: 49-53: (1991).
- Lane, D.P, Nature 358: 15-16: (1992).
- Donehower et al, Biochemic. Biophys. Acta1155: 181-182, (1993).