p53 Mutant polyclonal, anti-human

Background:
Acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis, the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression.

Alternative Name:
TP53, P53, Antigen NY-CO-13, BCC7, Cellular tumor antigen p53, LFS1, Mutant tumor protein 53, p53 tumor suppressor, P53, Phosphoprotein p53, Tp53, Transformation related protein 53, TRP53, Tumor protein p53, Tumor suppressor p53,

Application Dilution:
WB:1:500
IHC:1:100
FC:1:50-1:100
ICC:1:100-1:500

Specificity: p53 Mutant polyclonal antibody detects endogenous levels of p53 Mutant protein.

Immunogen:
Recombinant protein

MW: 53 kDa

Swiss-Prot: P04637(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.