p53 polyclonal, anti-human

Quantity: 100 µl

Background:
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277).

Synonyms:
P53, BCC7, LFS1, TRP53

Isotype: Rabbit Polyclonal

Immunogen:
Recombinant p53 protein

Form: Serum containing 0.1% sodium azide

Specificity:
The polyclonal antibody shows a nuclear staining pattern with wild-type and most mutant forms of p53 protein.

Reactivity: human

Applications: Immunofluorescence, Immunoblotting, Immunohistochemistry of frozen and paraffin sections, no proteolytic treatment required

Working DIlution: 1:25 – 1:50

Positive Control:
Breast carcinoma

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
- Bartek, J. et al.(1990) Oncogene 5 : 893-899
- Hall, P.A. et al. (1991) Lancet pp338-513