p53 Polyclonal Antibody

Background:
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).

Research Area: Cancer, Cell Biology, Epigenetics and Nuclear Signaling

Synonyms:
BCC7, LFS1, P53, TRP53, p53, TP53, BMFS5

Immunogen: Recombinant fusion protein of human p53 (NP_000537.3).

Swissprot: P04637
Gene ID: 7157

Calculated MW: 23-43 kDa
Observed MW: 53 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Concentration: 1 mg/mL
Dilution: "WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200"

Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Citation(s):
https://pdfs.semanticscholar.org/ba96/13d024251d8089f8af0401d7a2322108c0b6.pdf