p62 Protein, C-Terminal Specific polyclonal, anti-human, rat, mouse

Quantity: 25 µl

Background:
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

Synonyms:
SQSTM1, Sequestosome, Ubiquitin-binding protein, p60, p62, A170, OSIL, PDB3, ZIP3, p62B

Isotype: Guinea pig Polyclonal

Immunogen:
C-terminal domain (20 amino acids: C-NYD IGA ALD TIQ YSK HPP PL) of human p62 protein, coupled with KLH. This peptide sequence is identical in human, monkey, bovine, mouse, and rat

Form: Stabilized Serum

Specificity:
Human 62 kD (p62) protein, present in intracytoplasmic inclusions (e.g. hyaline bodies) of hepatocellular carcinoma. P62 protein has been found in many tissues and cells, including lymphoid cells, serving probably a common cellular signal transduction mechanism. The antiserum stains also neurofibrillary tangles in the brain of patients suffering from Alzheimer's disease. Note: The p62 antigen might be susceptible to proteolytic degradation. We recommend to use protease inhibitors during sample preparation.

Reactivity: human, rat, mouse

Applications: Immunohistochemistry on frozen and paraffin sections (enhanced after microwave treatment), Cytological material, Immunoblotting

Working DIlution: 1:100 for immunohistochemistry; 1:1,000 for immunoblotting (ECL method)

Storage: 2-8C

References:
- Stumptner, C. et al. (1999) Am. J. Pathol. 154: 1701-1710
- Janig, E. et al. (2005) Eur J Cell Biol. 84: 329-339
- Kuusisto, E. et al. (2007) Neuropathology & Applied Neurobiology, pp 1-12
- Aishima S et al. (2010) Am J Clin Pathol 134: 457-465
- Itakura E et al. (2011) J Cell Bio 192: 17-27