PABPN1 polyclonal, anti-human, mouse

Background:
Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation.

Alternative Name:
PABP-2, Poly(A)-binding protein 2, PABII, PABPN1, Nuclear poly(A)-binding protein 1, OPMD, PAB2, PABII, PABP 2, pABP-2, PABP2, PABP2, PABPII, Pabpn1, poly(A) binding protein nuclear 1, Poly(A)-binding protein 2, Poly(A)-binding protein II, PolyA binding protein II, Polyadenylate-binding nuclear protein 1, Polyadenylate-binding protein 2,

Application Dilution:
WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
IP:1:10-1:50

Specificity: PABPN1 polyclonal antibody detects endogenous levels of PABPN1 protein.

Immunogen:
recombinant protein

MW: 49 kDa

Swiss-Prot: Q86U42(Human) Q8CCS6(Mouse)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.