PACAP 38 polyclonal, anti-human, rat, mouse

Quantity: 50 µl

Background:
This gene encodes a secreted proprotein that is further processed into multiple mature peptides. These peptides stimulate adenylate cyclase and increase cyclic adenosine monophosphate (cAMP) levels, resulting in the transcriptional activation of target genes. The products of this gene are key mediators of neuroendocrine stress responses. Alternative splicing results in multiple transcript variants.

Synonyms:
PACAP, ADCYAP1

Isotype: Rabbit Polyclonal

Immunogen:
Synthetic PACAP 38, conjugated to BSA

Form: Serum containing 0.1% sodium azide

Specificity:
Absorption with 10-100 ?g immunogen per ml diluted antiserum abolishes staining, while PRP, VIP, PHI, CRF, oxytocin and vasopressin do not. Some cross-reactivity with PACAP-27.

Reactivity: human, rat, mouse

Applications: Immunohistochemistry, suitable for frozen sections

Working DIlution: 1:40-1:80 immunofluorescence microscopy

Positive Control:
Stefanini-fixed frozen sections of rat duodenum or hypothalamus

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
- Hannibal, J. et al. (1995) Regulatory Peptides 55: 133-148
- Kato J et al. (1998) Acta Anat 163: 92-98
- Hamelink C et al. (2002) Proc Natl Acad Sci USA 99: 461-466