PADI4/PAD4 polyclonal, anti-human

Quantity: 100 µg

Background:
PADI4(Peptidylarginine Deiminase, Type IV), also known as PAD or PAD4, is a human protein which in humans is encoded by the PADI4 gene. Peptidylarginine deiminases, such as PADI4, make up a family of posttranslational modification enzymes that convert arginine residues to citrulline residues in the presence of calcium ion(Nakashima et al., 1999). Suzuki et al.(2003) found from sequence data that the PADI4 gene maps to 1p36. Cuthbert et al.(2004) described a process, deimination, that converts histone arginine to citrulline and antagonizes arginine methylation. They showed that PADI4 specifically deiminated arg2, arg8, arg17, and arg26 in the H3 tail. Deimination by PADI4 prevented arginine methylation by CARM1. Dimethylation of arginines prevented deimination by PADI4, although monomethylation still allowed deimination to take place. In vivo targeting experiments on an endogenous promoter demonstrated that PADI4 could repress hormone receptor-mediated gene induction. PADI4 was recruited to the pS2 promoter following hormone induction when the gene was transcriptionally downregulated, consistent with a repressive role for PADI4.

Description:
Rabbit IgG polyclonal antibody for Protein-arginine deiminase type-4 (PADI4) detection. Tested with WB in Human.

Synonyms:
EC 3.5.3.15 antibody, HL 60 PAD antibody, HL-60 PAD antibody, PAD 4 antibody, PAD antibody, PADI 4 antibody, PADI 5 antibody, PADI H protein antibody, Padi4 antibody, PADI4_HUMAN antibody, PADI5 antibody, PDI 4 antibody, PDI 5 antibody, PDI4 antibody, PDI5 antibody, Peptidyl arginine deiminase type IV antibody, Peptidyl arginine deiminase type V antibody, Peptidylarginine deiminase IV antibody, Protein arginine deiminase antibody, Protein arginine deiminase type 4 antibody, Protein arginine deiminase type IV antibody, Protein-arginine deiminase type IV antibody, Protein-arginine deiminase type-4 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence in the middle region of human PADI4(492-512aa ASPRSCYKLFQEQQNEGHGEA).

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.