PAICS polyclonal, anti-human, mouse, rat
€388.00
In stock
SKU
BS8226
Background:
Two successive steps in de novo purine biosynthesis are catalyzed by the enzymes 5-aminoimidazole ribonucleotide (AIR) carboxylase and 4-[(N-succinylamino)carbonyl]-5-aminoimidazole ribonucleotide (SAICAR) synthetase. In vertebrates, a bifunctional enzyme, ADE2 ( also designated PurCE/AIRC) catalyzes successive carboxylation and aspartylation steps of AIR to form SAICAR. ADE2 is transcribed from a 558 bp intergenic promoter region, which is bound by NRF-1 and Sp1 at sites within the 215-260 region. The intergenic region is an integrated bidirectional promoter and a novel initiator-like element plays a central role in coordinating expression of the divergently transcribed ADE2 and GPAT genes. The ADE2 mRNA levels increase approximately five to six fold in G1/S phase of the cell cycle over those in G0 phase in synchronized rat 3Y1 fibroblasts . The ADE2 gene encodes enzyme of AIRC at step 6 and SAICR synthetase at step 7 in de novo purine nucleotide synthesis . The ADE2 encoded enzyme has no ATP dependence and no common cofactors or metals are required for catalysis . Activities of ADE2 enzyme are found in lysates of human erythrocytes, thrombocytes and leukocytes and in homogenate of the stomach biopsy sample, but not in blood plasma and bile .
Alternative Name:
Multifunctional protein ADE2, PAICS, ADE2, AIRC, PAIS
Application Dilution: WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:10 - 1:100
Specificity: PAICS polyclonal antibody detects endogenous levels of PAICS protein.
Immunogen:
Recombinant full length Human PAICS.
MW: ~ 47 kDa
Swis Prot.: P22234
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
Two successive steps in de novo purine biosynthesis are catalyzed by the enzymes 5-aminoimidazole ribonucleotide (AIR) carboxylase and 4-[(N-succinylamino)carbonyl]-5-aminoimidazole ribonucleotide (SAICAR) synthetase. In vertebrates, a bifunctional enzyme, ADE2 ( also designated PurCE/AIRC) catalyzes successive carboxylation and aspartylation steps of AIR to form SAICAR. ADE2 is transcribed from a 558 bp intergenic promoter region, which is bound by NRF-1 and Sp1 at sites within the 215-260 region. The intergenic region is an integrated bidirectional promoter and a novel initiator-like element plays a central role in coordinating expression of the divergently transcribed ADE2 and GPAT genes. The ADE2 mRNA levels increase approximately five to six fold in G1/S phase of the cell cycle over those in G0 phase in synchronized rat 3Y1 fibroblasts . The ADE2 gene encodes enzyme of AIRC at step 6 and SAICR synthetase at step 7 in de novo purine nucleotide synthesis . The ADE2 encoded enzyme has no ATP dependence and no common cofactors or metals are required for catalysis . Activities of ADE2 enzyme are found in lysates of human erythrocytes, thrombocytes and leukocytes and in homogenate of the stomach biopsy sample, but not in blood plasma and bile .
Alternative Name:
Multifunctional protein ADE2, PAICS, ADE2, AIRC, PAIS
Application Dilution: WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:10 - 1:100
Specificity: PAICS polyclonal antibody detects endogenous levels of PAICS protein.
Immunogen:
Recombinant full length Human PAICS.
MW: ~ 47 kDa
Swis Prot.: P22234
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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