PAK1/2/3 (Phospho-S144/S141/S139) polyclonal, anti-human, mouse, rat
€428.00
In stock
SKU
BS94089
Catalog Number: BS94089
Size: 100 µl
Other Size: 50 µl
Isotype: rabbit IgG
Applications: WB, ICC/IF, IHC, IP, FC
Questions? Contact us!
Size: 100 µl
Other Size: 50 µl
Isotype: rabbit IgG
Applications: WB, ICC/IF, IHC, IP, FC
Questions? Contact us!
Background:
Three isoforms of serine/threonine kinases, designated αPAK p68, βPAK p65 and γPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The α, β and γPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on γPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with γPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.
Alternative Name:
PAK-1, PAK1, PAK-2, PAK2, PAK-3, PAK3,
Application Dilution:
WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Specificity: PAK1/2/3 (Phospho-S144/S141/S139) polyclonal antibody detects endogenous levels of PAK1/2/3 protein only when phosphorylated at S144/S141/S139.
Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Ser144 of human PAK1.
MW: 65 kDa
Swiss-Prot: O75914(Human) Q13153(Human) Q13177(Human) O88643(Mouse) Q61036(Mouse) Q8CIN4(Mouse) P35465(Rat) Q62829(Rat) Q64303(Rat)
Purification & Purity:
ProA affinity purified
Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
For research use only, not for use in diagnostic procedure.
Three isoforms of serine/threonine kinases, designated αPAK p68, βPAK p65 and γPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The α, β and γPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on γPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with γPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.
Alternative Name:
PAK-1, PAK1, PAK-2, PAK2, PAK-3, PAK3,
Application Dilution:
WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Specificity: PAK1/2/3 (Phospho-S144/S141/S139) polyclonal antibody detects endogenous levels of PAK1/2/3 protein only when phosphorylated at S144/S141/S139.
Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Ser144 of human PAK1.
MW: 65 kDa
Swiss-Prot: O75914(Human) Q13153(Human) Q13177(Human) O88643(Mouse) Q61036(Mouse) Q8CIN4(Mouse) P35465(Rat) Q62829(Rat) Q64303(Rat)
Purification & Purity:
ProA affinity purified
Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
For research use only, not for use in diagnostic procedure.
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