PAK2 polyclonal, anti-human, mouse, rat

Background:
Three recently identified isoforms of serine/threonine kinases, designated αPAK p68, βPAK p65 and γPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase STE20, involved in pheromone signaling. The α, β, and γPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is MEK kinase, an upstream effector of MEK4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP-dependent manner with Rac1 and Cdc42, they do not interact with Rho.

Alternative Name:
PAK-2, p27, p34, PAK2, C-t-PAK2, CB422, EC 2.7.11.1, Gamma PAK, Gamma-PAK, hPAK65, Kinase, p21 (CDKN1A) activated kinase 2, p21 (CDKN1A)-activated kinase 2a, p21 activated kinase 2, p21 protein (Cdc42/Rac)-activated kinase 2, p21 protein Cdc42 Rac activated kinase 2, p21-activated kinase 2, p21-activated kinase, 65-KD, p21-activated protein kinase I, p21CDKN1A activated kinase 2, p27, p34, p58, p65PAK, PAK 2, PAK-2, PAK-2p34, Pak2, PAK2, PAK65, PAKgamma, S6 H4 kinase, S6/H4 kinase, Serine threonine protein kinase PAK 2, Serine/threonine protein kinase PAK 2,

Application Dilution:
WB:1:500-1:1,000
IHC:1:100-1:500
ICC:1:100-1:500
FC:1:50-1:100

Specificity: PAK2 polyclonal antibody detects endogenous levels of PAK2 protein.

Immunogen:
Recombinant protein

MW: 58 kDa

Swiss-Prot: Q13177(Human) Q8CIN4(Mouse)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.