PARP (clone M3), anti-human

Background:
Poly [ADP-ribose] polymerase 1 (PARP-1) also known as NAD+ ADP-ribosyltransferase 1 or poly[ADP-ribose] synthase 1 is an enzyme that in humans is encoded by the PARP1 gene. PARP1 has a role in repair of single-stranded DNA (ssDNA) breaks. Knocking down intracellular PARP1 levels with siRNA or inhibiting PARP1 activity with small molecules reduces repair of ssDNA breaks. In the absence of PARP1, when these breaks are encountered during DNA replication, the replication fork stalls, and double-strand DNA (dsDNA) breaks accumulate.

Synonyms: ADPRT, ADPRT1, ADPRT1, ARTD1, pADPRT-1, PARP, PARP-1, PPOL

Cellular Location: Nucleus nucleolus

Immunogen:
Synthetic Peptide

Gene Symbol: PARP

Gene ID: 142

Swiss prot: P09874

Calculated MW: 116kDa

Recommended dilution:
WB 1:1000-3000, IHC 1:200-500,

Purity:
Saturated ammonium sulfate precipitation

Storage Buffer:
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:
Store at -20℃. Avoid freeze / thaw cycles.