PARP polyclonal, anti-human, mouse, rat

Background:
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability, cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.

Alternative Name:
PARP-1, ARTD1, ADPRT 1, PARP1, ADP ribosyltransferase (NAD+, poly (ADP ribose) polymerase), ADP ribosyltransferase diphtheria toxin like 1, ADP ribosyltransferase NAD(+), ADPRT 1, ADPRT, ADPRT1, ARTD1, msPARP, NAD(+) ADP ribosyltransferase 1, NAD(+) ADP-ribosyltransferase 1, pADPRT 1, pADPRT1, PARP 1, PARP, PARP-1, PARP1, PARP1, Poly (ADP ribose) polymerase 1, poly (ADP ribose) polymerase family, member 1, Poly [ADP-ribose] polymerase 1, Poly(ADP ribose) polymerase, poly(ADP ribose) synthetase, poly(ADP ribosyl)transferase, Poly[ADP ribose] synthetase 1, Poly[ADP-ribose] synthase 1, PPOL,

Application Dilution:
WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Specificity: PARP polyclonal antibody detects endogenous levels of PARP protein.

Immunogen:
recombinant protein

MW: 113/89 kDa

Swiss-Prot: P09874(Human) P11103(Mouse) P27008(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.