Paxillin (C-terminal) Blocking Peptide
€155.00
In stock
SKU
ECM-PX1165
Background:
Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells. Thus, phosphorylation paxillin at serine 178 may be important during cell migration.
Sequence: A synthetic peptide corresponding to amino acid residues in the C-terminal region of human Paxillin. This peptide sequence is highly conserved in rat and mouse paxillin, and is found in all three paxillin isoforms (α, β,and γ).
Specificity: The peptide is specifically recognized by anti-Paxillin (C-terminal) antibody (PP1161) in ELISA, and has been shown to block the reactivity of PP1161 during Western blot. In addition, the peptide is recommended for use in blocking PP1161 reactivity in immunocytochemistry.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells. Thus, phosphorylation paxillin at serine 178 may be important during cell migration.
Sequence: A synthetic peptide corresponding to amino acid residues in the C-terminal region of human Paxillin. This peptide sequence is highly conserved in rat and mouse paxillin, and is found in all three paxillin isoforms (α, β,and γ).
Specificity: The peptide is specifically recognized by anti-Paxillin (C-terminal) antibody (PP1161) in ELISA, and has been shown to block the reactivity of PP1161 during Western blot. In addition, the peptide is recommended for use in blocking PP1161 reactivity in immunocytochemistry.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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