PBP polyclonal, anti-rat, mouse

Quantity: 100 µg

Background:
PEBP1(Phosphatidylethanolamine-binding protein 1), also called PBP, RKIP, inhibits the phosphorylation and activation of MEK by RAF1. PEBP1 is identical to the phosphatidylethanolamine-binding protein(PBP) with a relative molecular mass of 23 kD. The PEBP1 gene is mapped on 12q24.23. PEBP1 coimmunoprecipitates with RAF1 and MEK from cell lysates and colocalizes with RAF1 when examined by confocal microscopy. PEBP1 overexpression interferes with the activation of MEK and ERK, induction of AP1-dependent reporter genes, and transformation elicited by an oncogenically activated RAF1 kinase. PEBP1 expression was rapidly upregulated during induction of chemotherapy-triggered apoptosis in human prostate and breast cancer cell lines, and maximal RKIP expression correlated perfectly with the onset of apoptosis by Chatterjee et al(2004). RKIP depletion decreased the mitotic index, the number of metaphase cells, traversal times from nuclear envelope breakdown to anaphase, and an override of mitotic checkpoints induced by spindle poisons.

Description:
Rabbit IgG polyclonal antibody for Phosphatidylethanolamine-binding protein 1 (PEBP1) detection. Tested with WB, IHC-P in Mouse, Rat.

Synonyms:
HCNP antibody, HCNPpp antibody, Hippocampal cholinergic neurostimulating peptide antibody, Neuropolypeptide h3 antibody, PEBP antibody, PEBP 1 antibody, PEBP-1 antibody, PEBP1 antibody, PEBP1_HUMAN antibody, Phosphatidylethanolamine binding protein antibody, Phosphatidylethanolamine binding protein 1 antibody, Prostatic binding protein antibody, Prostatic-binding protein antibody, R kip antibody, Raf kinase inhibitor protein antibody, Raf kinase inhibitory protein antibody, RKIP antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of mouse PBP(15-29aa QEVDEPPQHALRVDY), identical to the related rat sequence.A synthetic peptide corresponding to a sequence at the N-terminus of mouse PBP(15-29aa QEVDEPPQHALRVDY), identical to the related rat sequence.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: rat.
Predicted to work with: mouse.

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.