PCNA (clone PC), anti-human, mouse, rat
€354.00
In stock
SKU
ARP-10-M1083
Catalog Number: 10-M1083
Size: 100 µg
Isotype: Mouse IgG2a
Size: 100 µg
Isotype: Mouse IgG2a
Background:
Proliferating cell nuclear antigen(PCNA) was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferative state of the cell. PCNA/cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. PCNA gene is present in single copy and has 6 exons. It spans 4,961 bp. Synthesis of the nuclear protein cyclin and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. PCNA controls establishment of sister chromatid cohesion during S phase.
Decription:
Mouse IgG monoclonal antibody for PCNA, proliferating cell nuclear antigen (PCNA) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, mouse, rat. No cross reactivity with other proteins.
Synonyms: Cyclin, DNA POLYMERASE DELTA AUXILIARY PROTEIN
Clone: PC
Isotype: Mouse IgG2a
Immunogen: Protein A fusion protein.
Reactivity: human, mouse, rat
Form: Lyophilized; Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Applications: WB, IHC-P, IHC-F, ICC
Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.
Proliferating cell nuclear antigen(PCNA) was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferative state of the cell. PCNA/cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. PCNA gene is present in single copy and has 6 exons. It spans 4,961 bp. Synthesis of the nuclear protein cyclin and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. PCNA controls establishment of sister chromatid cohesion during S phase.
Decription:
Mouse IgG monoclonal antibody for PCNA, proliferating cell nuclear antigen (PCNA) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, mouse, rat. No cross reactivity with other proteins.
Synonyms: Cyclin, DNA POLYMERASE DELTA AUXILIARY PROTEIN
Clone: PC
Isotype: Mouse IgG2a
Immunogen: Protein A fusion protein.
Reactivity: human, mouse, rat
Form: Lyophilized; Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Applications: WB, IHC-P, IHC-F, ICC
Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.
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