PCNA Polyclonal Antibody

Background:
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.

Research Area: Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Tags and Cell Markers

Synonyms:
ATLD2, cb16, Cyclin, DNA polymerase delta auxiliary protein, etID36690.10, fa28e03, fb36g03, HGCN8729, MGC8367, Mutagen-sensitive 209 protein, OTTHUMP00000030189, OTTHUMP00000030190, PCNA, Pcna/cyclin, PCNA, PCNAR, Polymerase delta accessory protein, Proliferating cell nuclear antigen, wu:fa28e03, wu:fb36g03

Immunogen: Recombinant human Proliferating cell nuclear antigen protein

Swissprot: P12004
Gene Accession: BC000491

Calculated MW: 29 kDa
Observed MW: 34 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.05% Proclin300 and 50% glycerol, pH7.4.

Concentration: 2 mg/mL
Dilution: WB 1:500-1:1000, IHC 1:50-1:100

Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Alternative for product with catalog number:
sc-65598,sc-71858,sc-25280,sc-53407,sc-53408,sc-56

Citation(s):
https://pubmed.ncbi.nlm.nih.gov/32461192/