PCNA Polyclonal Antibody

Background:
Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells.

Research Area: Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Tags and Cell Markers

Synonyms:
ATLD2, cb16, Cyclin, DNA polymerase delta auxiliary protein, etID36690.10, fa28e03, fb36g03, HGCN8729, MGC8367, Mutagen-sensitive 209 protein, OTTHUMP00000030189, OTTHUMP00000030190, PCNA, Pcna/cyclin, PCNA, PCNAR, Polymerase delta accessory protein, Proliferating cell nuclear antigen, wu:fa28e03, wu:fb36g03

Immunogen: Recombinant protein corresponding to Mouse PCNA

Swissprot: Q12004,P17918,P04961

Calculated MW: 36 kDa
Observed MW: 36 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH7.4

Concentration: 470 μg/mL
Dilution: WB 1:500-1:2000, IHC 1:500-1:1000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.