PD-1 (clone RMP1-14.1), anti-mouse In Vivo Recombinant Monoclonal Antibody

Background:
The rat anti-mouse PD-1 monoclonal antibody reacts with the mouse PD-1 protein (CD279 or programmed death-1) encoded by the mouse pdcd1 gene, a member of the CD28 family of the Ig superfamily. PD-1 has two ligands, PD-L1 and PD-L2, both of which belong to the B7 family. It has been shown that in mouse models of melanoma, tumor growth can be transiently arrested via treatment with the anti-mouse PD 1 antibody and the anti-mouse PD L1 antibody which block the interaction between the PD-L1 protein and its receptor PD-1 protein. The RMP1-14 antibody blocks the binding of both the mouse PD-L1 protein and the mouse PD-L2 protein to the mouse PD-1 protein.

Immunogen:
The original rat hybridoma (clone name: RMP1-14) was generated by immunizing Sprague Dawley rats with mouse PD-1-transfected BHK cells and using a P3U1 myeloma as the fusion partner.

Specificity/Sensitivity :
The in vivo grade recombinant rat monoclonal antibody (clone: RMP1-14) specifically binds to mouse PD-1.

Purity: >95% by SDS-PAGE

Endotoxin level: <1 EU/μg by LAL test

Application: IHC, Flow-Cyt, In vitro and in vivo functional assays

Formulation: 0.2 μM filtered solution of 1x PBS

Storage and Expiration:
Ship at 2-8°C, when receipt, aliquot and store at-20°Cto -70°Cfor one year. Avoid repeated freeze and thaw cycles.