PD-L1 (clone 10F.9G2.1), anti-mouse In Vivo Recombinant Monoclonal Antibody

Background:
The rat anti-mouse PD-L1 monoclonal antibody 10F.9G2 (rat IgG2b kappa) reacts with the mouse PD-L1 protein (programmed death ligand-1, B7-H1 or CD274), a member of the B7 family of the Ig superfamily. PD-1 has two ligands, PD-L1 and PD-L2. It has been shown that in mouse models of melanoma, tumor growth can be transiently arrested via treatment with the anti-mouse PD-1 and anti-mouse PD-L1 antibodies which block the interaction between the PD-L1 protein and its receptor PD-1 protein. The 10F.9G2 monoclonal antibody blocks the binding of the mouse PD-L1 protein to the mouse PD-1 protein.

Immunogen:
The original rat hybridoma (clone name: 10F.9G2) was generated by immunizing rats with the mouse PD-L1 cDNA and mouse PD-L1 CHO transfectants.

Purity: >95% by SDS-PAGE

Endotoxin level: <1 EU/μg by LAL test

Application: IHC, Flow-Cyt, In vitro and in vivo functional assays

Formulation: 0.2 μM filtered solution of 1x PBS

Storage and Expiration:
Ship at 2-8°C, when receipt, aliquot and store at-20°Cto -70°Cfor one year. Avoid repeated freeze and thaw cycles.