PDGF-BB polyclonal, anti-human
€0.00
In stock
SKU
AC-ABG10457
Catalog Number: AC-ABG10457
Size(s): 50 µg, 100 µg
Host: Rabbit
Applications: WB, IHC, E
Datasheet
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Size(s): 50 µg, 100 µg
Host: Rabbit
Applications: WB, IHC, E
Datasheet
Request Information
Source:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPDGF-BB. Anti-Human PDGF-BB specific antibody was purified by affinity chromatography employing immobilized hPDGF-BB matrix.
Western Blot:
To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA:
To detect hPDGF-BB by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Human PDGF-BB (60-267BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPDGF-BB.
Immunohistochemistry:
This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 μg/ml overnight at 4°C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hPDGF-BB (2.5 ng/ml), a concentration of 0.03-0.04 μg/ml of this antibody is required.
Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPDGF-BB. Anti-Human PDGF-BB specific antibody was purified by affinity chromatography employing immobilized hPDGF-BB matrix.
Western Blot:
To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA:
To detect hPDGF-BB by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Human PDGF-BB (60-267BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPDGF-BB.
Immunohistochemistry:
This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 μg/ml overnight at 4°C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hPDGF-BB (2.5 ng/ml), a concentration of 0.03-0.04 μg/ml of this antibody is required.
Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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