PDGFRβ (phospho Y740) polyclonal, anti-human, rat

Background:
Platelet Derived Growth Factor (PDGF) is a mitogen for mesenchyme- and glia-derived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms, PDGF-AA, PDGF-AB, and PDGF-BB. These three isoforms bind with different affinities to two receptor types, a and b, which are endowed with protein tyrosine kinase domains and undergo either homo- or heterodimerization as a consequence of ligand binding. Ligand stimulation of PDGFR-b leads to autophosphorylation at Tyr-857, which is the major autophosphorylation site, and Tyr-751, which is the major in vitro phosphorylation site. Autophosphorylation of Tyr-751, which lies in the kinase insert region, is required for binding of phosphatidylinositol-3 kinase to the receptor. These auto-phosphorylation events largely contribute to signal transduction through the PDGF receptor.

Alternative Name:
PDGF-R-beta, PDGFR-beta, PDGFR-1, PDGFRB, Pdgfrb, Beta platelet derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD 140B, CD140 antigen-like family member B, CD140b, CD140b antigen, IBGC4, IMF1, JTK12, OTTHUMP00000160528, PDGF R beta, PDGF-R-beta, PDGFR 1, PDGFR, PDGFR beta, PDGFR1, PDGFRB, PGFRB, Platelet derived growth factor receptor 1, Platelet derived growth factor receptor beta, Platelet derived growth factor receptor beta polypeptide,

Application Dilution:
WB:1:1,000
ICC:1:50-1:200

Specificity: PDGFRβ (phospho Y740) polyclonal antibody detects endogenous levels of PDGFRβ protein only when phosphorylated at Y740.

Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Tyr740 of human PDGF Receptor beta.

MW: 190 kDa

Swiss-Prot: P05622(Mouse) Q05030(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.