PDK1 polyclonal, anti-human, mouse, rat

Background:
Mitochondrial pyruvate dehydrogenase (PDH) catalyzes the oxidative decarboxylation of pyruvate and plays a central role in the regulation of homeostasis of carbohydrate fuels in mammals. PDH activity is controlled by a phosphorylation/dephosphorylation cycle, phosphorylation leading to inactivation and dephosphorylation leading to reactivation of PDH. The phosphorylation of PDH is catalyzed by pyruvate dehydrogenase kinase (PDK), the activity of which is stimulated by the products of PDH catalysis. PDK1 consists of alpha and beta subunits, the kinase activity resides in the alpha subunit. Three PDK isoenzymes have been identified in humans (PDK1, 2 and 3) and two have been identified in rodent (PDK1 and 2).

Alternative Name:
PDH kinase 1, PDK1, PDHK1,

Application Dilution:
WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Specificity: PDK1 polyclonal antibody detects endogenous levels of PDK1 protein.

Immunogen:
recombinant protein

MW: 49 kDa

Swiss-Prot: Q15118(Human) Q8BFP9(Mouse) Q63065(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.