PDPK1 (phospho-S241) polyclonal, anti-human, mouse, rat
€328.00
In stock
SKU
BS4155
Background:
PDK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of hosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.
Alternative Name:
3-phosphoinositide-dependent protein kinase 1, hPDK1, PDPK1, PDK1
Application Dilution: WB: 1:500~1:1000, IHC: 1:50~1:200
Specificity: p-PDPK1 (S241) polyclonal antibody detects endogenous levels of PDPK1 protein when phosphorylated at Ser241.
Immunogen:
Synthetic phosphopeptide derived from human PDPK1 around the phosphorylation site of Serine 241.
MW: ~ 63 kDa
Swis Prot.: O15530
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
PDK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of hosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.
Alternative Name:
3-phosphoinositide-dependent protein kinase 1, hPDK1, PDPK1, PDK1
Application Dilution: WB: 1:500~1:1000, IHC: 1:50~1:200
Specificity: p-PDPK1 (S241) polyclonal antibody detects endogenous levels of PDPK1 protein when phosphorylated at Ser241.
Immunogen:
Synthetic phosphopeptide derived from human PDPK1 around the phosphorylation site of Serine 241.
MW: ~ 63 kDa
Swis Prot.: O15530
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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