PDPK1 polyclonal, anti-human, mouse, rat

Background:
PDPK1 (3-phosphoinositide dependent protein kinase 1), also known as PDK1, PDPK2, PDPK2P or PRO0461, is 556 amino acid ubiquitously expressed protein that localizes to the cell membrane, cytoplasm and nucleus. Acting as a master kinase, PDPK1 phosphorylates and activates a subgroup of the AGC family of protein kinases. PDPK1 is involved in mediating signal transduction for controlling proliferation, survival, and growth of developing pancreatic beta cells, regulating Ca2+ uptake and Ca2+-activated K+ channels of mast cells, regulation of chemotaxis and motility of vascular endothelial cells, cardiac homeostasis, and thymocyte development. Belonging to the protein kinase superfamily, PDPK1 contains a PH domain, which play an essential role in homodimerization, localization and nuclear import of PDPK1, and a protein kinase domain. PDPK1 exists as five alternatively spliced isoforms and is encoded by a gene located on human chromosome 16p13.3.

Alternative Name:
hPDK1, 3 phosphoinositide dependent protein kinase 1, 3-phosphoinositide-dependent protein kinase 1, hPDK 1, hPDK1, MGC20087, MGC35290, OTTHUMP00000159109, OTTHUMP00000159110, OTTHUMP00000174525, PDK1, Pdpk1, PDPK1, PDPK2, PDPK2P, PkB kinase, PkB kinase like gene 1, PkB like 1, PRO0461, Protein kinase,

Application Dilution:
WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200

Specificity: PDPK1 polyclonal antibody detects endogenous levels of PDPK1 protein.

Immunogen:
recombinant protein

MW: 63/48 kDa

Swiss-Prot: O15530(Human) Q9Z2A0(Mouse) O55173(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.