Perforin polyclonal, anti-human

Quantity: 100 µg

Background:
PRF1, also known as Perforin-1, is a protein that in humans is encoded by the PRF1 gene. It is mapped to 10q22.1. PRF1 is a cytolytic protein found in the granules of Cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, PRF1 inserts itself into the target cell's plasma membrane, forming a pore. The lytic membrane-inserting part of perforin is the MACPF domain. This region shares homology with cholesterol-dependent cytolysins from Gram-positive bacteria. PRF1 has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. PRF1 is thought to act by creating holes in the plasma membrane which triggers an influx of calcium and initiates membrane repair mechanisms. These repair mechanisms bring perforin and granzymes into early endosomes.

Description:
Rabbit IgG polyclonal antibody for Perforin-1 (PRF1) detection. Tested with WB in Human.

Synonyms:
Cytolysin antibody, FLH2 antibody, HPLH2 antibody, Lymphocyte pore forming protein antibody, Lymphocyte pore-forming protein antibody, MGC65093 antibody, P1 antibody, PERF_HUMAN antibody, perforin 1 (pore forming protein) antibody, Perforin 1 antibody, Perforin 1 precursor antibody, Perforin 1 preforming protein antibody, Perforin-1 antibody, PFP antibody, PGFL antibody, PIGF antibody, PIGF-2 antibody, PLGF antibody, Pore forming protein antibody, PRF 1 antibody, PRF1 antibody, SHGC-10760 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
E.coli-derived human Perforin recombinant protein (Position: E175-W555). Human Perforin shares 68% amino acid (aa) sequence identity with both mouse and rat Perforin.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.