Perilipin 5 polyclonal, anti-human, bovine, mouse, rat

Quantity: 25 µl

Background:
Members of the perilipin family, such as PLIN5, coat intracellular lipid storage droplets and protect them from lipolytic degradation

Synonyms:
MLDP, LSDA5, LSDP5, OXPAT, PAT-1

Isotype: Guinea Pig Polyclonal (GP31)

Immunogen:
Synthetic Peptide (C-terminal, aa 451-463 of human MLDP). Sequence: CPVKHTLMPELDF

Form: Stabilized Serum

Specificity:
Polypeptide reacting: MLDP (Myocardial Lipid Droplet Protein) also described as OXPAT/PAT-1 and and perilipin 5 / PLIN5). Molecular Weight (Mr): 50,844 (from aa sequence data); apparent Mr : 52,000 (SDS-PAGE). MLDP pertains to the PAT-family proteins, covering the surface of cytoplasmic lipid droplets and sharing a homologous domain called PAT. No cross reactivity with additional PAT proteins include adipophilin (ADRP), perilipin, and TIP47 which are expressed in differentiation-related stages of lipid metabolism

Reactivity: human, bovine, mouse, rat

Applications: Immunohistochemistry on cells and frozen sections, Immunoblotting (Western, ECL)

Working DIlution: Immunohistochemistry: 1:100 to 1:200; Western Blotting: 1:2,000 (ECL)

Positive Control:
MLDP is positively detected in heart, skeletal muscle. Reactivities in Cultured Cell Lines: HaCat (human), SV80 (human), 3T3-L1 (Mouse).

Storage: 2-8C

References:
- Wolins, NE. et al. (2006) Diabetes 55: 3418-3428
- Wolins, NE. et al. (2006) FEBS Letter 580: 5484-5491
- Yamaguchi, T et al. (2006) J Biol Chem 281: 14232-14240