Perilipin polyclonal, anti-human, bovine, rat, mouse

Quantity: 25 µl

Background:
Perilipin, also known as lipid droplet-associated protein or PLIN, is a protein that, in humans, is encoded by the PLIN gene. Perilipin is a protein that coats lipid droplets in adipocytes, the fat-storing cells in adipose tissue. Perilipin acts as a protective coating from the body's natural lipases, such as hormone-sensitive lipase, which break triglycerides into glycerol and free fatty acids for use in metabolism, a process called lipolysis.

Synonyms:
PERI, PLIN, FPLD4

Isotype: Guinea pig Polyclonal

Immunogen:
Duplicated N-terminus of perilipin (aa 1-20; cf. Greenberg et al. 1992, JBC 266, 11341-11346)

Form: Stabilized Serum

Specificity:
Specific for human, bovine, rat, mouse perilipin. The antiserum reacts specifically with perilipins (A and B) located at the surface of intracellular storage lipid droplets present e.g. in the adrenal gland, adipocytes of white and brown adipose tissue and cultured cells such as 3T3-L1 adipocytes and cultured steroidogenic adrenal cortical and Leydig cells. It also is a useful pathological marker since perilipin becomes expressed de novo in hepatocyte steatogenesis. The antiserum does not cross-react with ADRP (adipophilin) or TIP47 proteins (additional members of the PAT-family).

Reactivity: human, bovine, rat, mouse

Applications: Immunohistochemistry, suitable for frozen & paraffin embedded tissue after microwave treatment, Immunoblotting (Western), Cytological material

Working DIlution: Immunohistochemistry: 1:100 to 1:200; Western Blotting: 1:2,000 (ECL)

Storage: 2-8C

References:
- Heid HW et al. (1998) Cell Tissue Res 294: 309-321
- Ryden M et al. (2004) Biochem Biophys Res Comm 318: 168-175
- Forcheron F et al. (2005) Aeterioscler Thromb Vasc Biol 25: 1-7
- Yamaguchi T et al. (2006) Mol Cell Biochem 284: 167-173
- Straub BK et al. (2008) Hepatology 47: 1936-1946
- Straub BK et al. (2010) Modern Pathology 23: 480-492