Periplakin polyclonal, anti-human, bovine

Quantity: 25 µl

Background:
The protein encoded by PPL gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling.

Synonyms:
PPL

Isotype: Guinea pig Polyclonal

Immunogen:
Synthetic peptides of human periplakin (P1a/b, P2a/b, P3a/b), coupled to KLH

Form: Stabilized Serum

Specificity:
Specific for periplakin, a 190 kD protein localized e.g. in epidermis (cornified envelope) and cortical lens fiber cells

Reactivity: human, bovine

Applications: Immunofluorescence microscopy on frozen sections, Immunoblotting (western, ECL), Immunoprecipitation

Working DIlution: 1:50 - 1:100 for Immunofluorescence microscopy applications; 1:1000 - 1:2000 for Immunoblotting (western, ECL)

Storage: 2-8C

References:
- Straub BK. et al. (2003) Eur J Cell Sci 116: 4985-4995